Protoplast Technology

Protoplast Regeneration and Protoplast Fusion

Fytagoras fully explores the potential of plant cell protoplasts regeneration for recalcitrant crops. The availability of reliable protocols for protoplast regeneration is urgent in view of the progress made in gene editing technology for crop improvement the last years. In addition, protoplast fusion opens the way for new breeding strategies.

Plant breeders looking to explore the potential of protoplast regeneration should contact Fytagoras. Fytagoras specializes in researching the process and can provide valuable insight into the most effective methods for regenerating protoplasts from crop plants. Furthermore, the protocols developed can be tailored to the specific needs of the plant breeder, giving them the best chance of success. With the help of Fytagoras breeders can unlock the potential of this powerful tool in crop improvement.

Plant cell protoplasts

Benefits of Proplast Regeneration in Your Breeding Programme

Essential for application of Gene Editing, CRISPR/Cas9 technology

Options for protoplast fusions for introducing Cellular Male Sterility

Options for interspecific crossing via protoplast fusions

Options for improved propagation efficiency

Quicker anticipation to customer requests

Protoplast fusion and sorting

Fytagoras fully explores the use of plant cell protoplasts for advanced breeding, including protoplast regeneration, fusion, handling, and sorting. In the application of an alternating electrical field, it is possible to line-up plant cell protoplasts. These protoplasts trains are good targets to accomplish protoplast fusion of selected protoplasts by a strong electrical field pulse.  The video shows the formation of a protoplast train under an alternating electrical field. This phenomenon can be exploited for protoplast translocation, sorting, fusion and identification.

Examples of protoplast technology