Protocol development for the production of doubled haploid plants
In plant breeding the addition of desired properties to stable varieties is very labour intensive and time consuming, especially in slow growing species. Therefore, techniques that speed up the breeding process are very interesting. As the gametes, the reproductive cells, only contain half of the genetic material (chromosomes) of the parent plant, they are excellent for the production of new plants with a pure genetic background (homozygotes). By careful treatment of isolated reproductive cells and the application of different stress and growth conditions, these cells can double their genetic material and can be stimulated to divide and form multicellular structures, embryos and plants. For every species and often also for every variety the optimal conditions for the generation of doubled haploid must be selected and optimized. The success of our approach has been proven for a wide variety of crops.
Fytagoras in Leiden has many years of experience and a great track record in the development of protocols for the production of doubled haploid plants from many crops and varieties.
The strong point is the phasing approach in combination with practical experience in donor plant selection and treatment selection. Our program has three phases. In the first phase the quality, vitality and responsiveness of the cells will be determined. In the next phase the basic protocol development is done, while in the third phase the protocol will be optimized for efficiency.
After the protocol development Fytagoras can also be engaged in implementing the protocol in the daily use in practice at the customers premises.
Download the presentation “Fytagoras’ approach in DH production”