The practical success of doubled haploid (DH) plant production relies heavily on protocol efficiency. Fytagoras excels at developing DH protocols for notoriously challenging species, specifically optimizing them for seamless integration into breeding company laboratories.
Because DH production involves a complex sequence of steps, Fytagoras aims to refine every stage of the process to maximize efficiency and applicability. Key research areas include:
- Reducing the required number of donor plants.
- Simplifying flower bud collection, selection, and sterilization.
- Enabling fast, large-scale microspore isolation with high induction rates.
- Minimizing embryo transfers and medium refreshments.
- Boosting germination rates and simplifying plantlet handling.
- Automating quality and ploidy level controls.
- Securing greenhouse transfer protocols to minimize plant loss.
Optimizing these stages should yield streamlined protocols that significantly reduce resources, including greenhouse space, plastics, media, and labor. Furthermore, refined methods are more sustainable, require only basic tissue culture training to execute, and substantially shorten the total timeline from donor plant cultivation to established DH plants in the greenhouse.
