Potato genetic pool determines variation in true doubled haploid formation efficiency

From heterozygous diploid potato plants, we produce true homozygous doubled haploids  via androgenesis. Efficiency, measured as the percentage of true doubled haploids compared to haploids and diploid heterozygotes, varies by gene pool. Data from three pools show that gene pool 2 yields over 60% true DH plants, while pools 1 and 3 reach only about 10% and 20%, respectively. These results highlight strong variation in spontaneous chromosome doubling in microspore-based DH protocols for potato. Fytagoras continues to optimize methods to ensure high efficiency across all plant varieties.

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